My STEM Story: Krishna Parikh

Krishna Parikh analyzed DNA sequences for her Congressional Award Personal Development goal.

While striving to achieve The Congressional Award Gold Medal, I tapped into my interest in genetic engineering, research, and science in general.

As part of the Authentic Science Research class at my high school, I was able to participate in hands-on laboratory work. We correlated with the Waksman Student Scholars Program at Rutgers, following their procedures and techniques.

The current project is to focus on the DNA sequences of the duckweed plant Landoltia punctata and to further analyze the genes they are associated with. Duckweed is of interest because of its use in bioremediation and its potential in being a biofuel. Through this project, it is hoped that the genes compare to those found in other species.

Each of the students, including me, participate by isolating and sequencing genes from a cDNA library. These sequences have not been determined yet, so if a student successfully analyzes one, it will be published in GenBank. By comparing these sequences to similar ones of other eukaryotes, we are able to understand the evolutionary relationship between the two. This was accomplished through multiple lab days and through multiple hours sequencing on the DNA Sequencing Analysis Program (DSAP).

To increase these newly acquired skills, I attended a meeting at GenSpace in Brooklyn, New York. The meeting pertained to Optogenetics, which is a genetic tool that makes cells responsive to light.

Through the various experimentation, the end goal is to perfect an optogenetic system in which different colors of light shine onto a petri dish and cause the bacteria to respond. This response can lead to bacteria photographs with high resolution and the control of gene expression and useful enzymes, such as Taq Polymerase, in 2D.

By spending time with professionals and learning in the lab, I perfected my pipetting skills and better understood proper lab technique. I also learned how to transfer bacteria, making it anti-resistant to antibiotics such as ampicillin and kanamycin.

The most unfamiliar concept I worked with, regarding the optogenetic systems, was the on-off ratios. This ratio determines the resolution of a bacterial photograph produced by comparing the parts of the petri dish where light was directed to the parts where it was not. It is measured in Miller Units and requires the usage of a spectrometer that tells us the wavelengths of the produced light.

As the youngest in this class, it is difficult to fully understand what is going on at all times. This allows me to challenge myself and forces me to think beyond my comfort zone. I still attend these classes monthly, and I cannot wait to see the developments made.

Learn more about The Congressional Award STEM Stars program.